Elimination of Clonogenic Stem Cells From Human Multiple Myeloma Cell Lines
نویسندگان
چکیده
The monoclonal antibody (MoAb) MM4 reacts with human multiple myeloma (MM) cell lines and bone marrow from patients with plasma cell dyscrasias but not with normal peripheral blood or bone marrow cells. Treatment with MM4 and rabbit complement (C’) was cytotoxic to the plasma cell-derived cell lines GM 1312. RPMI 8226, and ARH-77. as demonstrated by chromium release microcytotoxicity and trypan blue exclusion assays. The same treatment eliminated >99% of clonogenic myeloma stem cell colony formation of these cell lines. with less than 20% inhibition of normal human bone marrow pleuripotent progenitor colony formation in vitro. As an experimental
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متن کاملElimination of clonogenic stem cells from human multiple myeloma cell lines by a plasma cell-reactive monoclonal antibody and complement.
The monoclonal antibody (MoAb) MM4 reacts with human multiple myeloma (MM) cell lines and bone marrow from patients with plasma cell dyscrasias but not with normal peripheral blood or bone marrow cells. Treatment with MM4 and rabbit complement (C') was cytotoxic to the plasma cell-derived cell lines GM 1312, RPMI 8226, and ARH-77, as demonstrated by chromium release microcytotoxicity and trypan...
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